Expression of cholesterogenic enzymes at 15 week of age.

The Taqman qPCR assay was used to measure the mRNA expression of the cholesterogenic enzymes in the cortex of 15 week old wild type (WT), Sirt2HET (HET), Sirt2KO (KO), R6/2, Sirt2HETxR6/2 (R6/2 HET) and Sirt2KOxR6/2 (R6/2 KO) mice. Values were normalised to the housekeeping genes Atp5b and Canx and expressed as fold change of WT ± SEM. n≥7/genotype.