Figure_7.tif (927.01 kB)
Expression of PC5 during fin regeneration.
figure
posted on 2013-02-21, 00:45 authored by Abdel-Majid Khatib, Rachid Lahlil, Nathalie Scamuffa, Marie-Andrée Akimenko, Sylvain Ernest, Abdderahim Lomri, Claude Lalou, Nabil G. Seidah, Bruno O. Villoutreix, Fabien Calvo, Geraldine Siegfried(A) Total RNA was isolated from fins (15-20 fins per time point) and analyzed by real-time PCR using specific primers for zebrafish PC5 or β-actin. Results are shown in the bar graph and are expressed as the ratio of the indicated transcripts relative to control (0 dpa). Results are shown as means ± S.E. of three experiments performed in triplicate. (B) Immunofluorescence analysis revealed that PC5 is expressed in all area of the regenerating fin and low signal was observed on the vessels (red signal, 25× objective).
