Expression of Dl in IFMs of 24 h APF pupae.

<p>Expression of Dl was detected with a anti-Dl antibody in wild-type B, B′–C,C') and <i>1151-Gal4, UAS-RNAiMef2</i> (E, E′–F,F') 21 h APF pupae. A'–F' show magnified areas indicated by the dotted squares in A–F respectively. Muscles are visualized with DAPI staining by the specific arrangement of their nuclei (asterisks in A, D, A′, D′; muscles are located between the two dotted lines in A′ and D′). Large nucleus (n in A′) is a larval template nucleus. Myoblasts are located between muscles (m in A′, D′). In wild type pupae, Dl is detected in myoblasts and developing fibers (B, B′; overlay in C, C′). Expression levels in myoblasts and fibers are close (B′). Overexpression of a <i>RNAi-Mef2</i> construct in myoblasts and fibers using the <i>1151-Gal4</i> driver (D–F, D′–F′) lowers Dl expression in developing fibers compared to myoblasts (E′, compare m to asterisks) and in <i>1151-Gal4</i>, <i>UAS-RNAiMef2</i> muscles compared to wild type muscles (compare E′, asterisks to B′, asterisk).</p>