Figure_4.tif (1.18 MB)
Exploration of annealing temperatures.
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posted on 2013-02-20, 02:35 authored by Hirokazu Toju, Akifumi S. Tanabe, Satoshi Yamamoto, Hirotoshi SatoThree ascomycete and four basidiomycete species were subjected to amplification of the (a) ITS1 region (ITS1-F_KYO–ITS2_KYO2), (b) ITS2 region (ITS3_KYO2–ITS4), and the (c) entire ITS region (ITS1-F_KYO2–ITS4) at four annealing temperatures (electrophoresed on 2.2% agarose gels). See Table 2 for the abbreviations of fungal specimens. N, negative control.
