Experimental design.

<p>(<b>A</b>) C57BL/6 mice were separated into four experimental groups that were euthanized on day 5 (5d) or day 21 (21d) post infection (p.i.): noninfected group (control<sub>5d</sub>); mice infected with 10<sup>6</sup> infected red blood cells euthanized on day 5 p.i. (infected<sub>5d</sub>); noninfected mice that received treatment with antimalarials (control+treated<sub>21d</sub>); and the <i>Plasmodium berghei</i> ANKA (PbA)-infected group treated with antimalarials and euthanized on day 21 p.i. (infected+treated<sub>21d</sub>). (<b>B</b>) Additional experimental groups that were euthanized on day 21 underwent or not a second kidney insult induced with intraperitoneal injections of bovine serum albumin (BSA): noninfected group (control<sub>21d</sub>); noninfected mice with BSA-induced renal injury (control+BSA<sub>21d</sub>); noninfected mice that received treatment with antimalarials and subsequent BSA injections (control+treated+BSA<sub>21d</sub>); PbA-infected mice treated with antimalarials (infected+treated<sub>21d</sub>); and PbA-infected mice that were treated and subsequently underwent a second kidney insult (infected+treated+BSA<sub>21d</sub>). (<b>C</b>) Parasitemia was determined from blood smears stained with Diff-Quick in all infected groups on day 1 (<b>A</b>), day 5 (<b>B</b>), day 8 (<b>C</b>), and day 21 (<b>D</b>) p.i.</p>