Exogenous BMP2 promotes increase of PHD2 protein level even under hypoxia by dowregulating FKBP38.

(A,B) Representative western blot analyses of PHD2 and FKBP38; GBM-derived cells, initially expanded in 2% oxygen were treated as described in Fig. 4. Also, some GBM-derived cells were treated with 10 ng/ml BMP2 for longer time (72 hrs) (B). (C,E) Bar graphs showing mean intensity of PHD2 and FKBP38 proteins normalized to control at 2% oxygen (corresponding to the 0 base line)±S.E.M. comparing 6 different tumors, n = 3 for each tumor. (D,F) QRT-PCR analyses of PHD2 and FKBP38, normalized to GUSB and then calibrated to 2% oxygen control (ΔΔCt Method), mean±S.E.M. comparing 3 different GBM, n = 3 for each tumor.