Examples of images collected with the InCell imaging system for the different treatments used with OE21 cells treated with 3µM Temoporfin for 24 hours before illumination and fixed at 4 four hours post illumination in 4% paraformaldehyde.

Cells were labeled with Hoechst–nucleus (blue at 345 excitation, 435 emission), phalloidin–F-actin (green at 475 excitation and 535 emission), mTHPC (red) was aquired at 560 excitation and 700 emission. Images were acquired by an InCell Analyzer automated microscope using a 10x objective (image size 0.897 mm×0.671 mm).