Examination of the minimal <i>Bmp3</i> upstream ECR (minECR) present in all RefSeq mammals.

<p>BLASTN (Version 2.2.26+) was used to align the <i>M. musculus</i>: <i>H. sapiens</i> CoreECR sequence to all sixteen available eutherian mammal RefSeq genomes in order to identify the <i>Bmp3</i> upstream ECR shared with <i>M. musculus</i>. Each full-length ECR was then aligned using MUSCLE in MEGA5 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0057840#pone.0057840-Tamura1" target="_blank">[64]</a>, identifying a minimal <i>Bmp3</i> upstream ECR (minECR) with a consensus length of 297 nt. The mean identity to the consensus minECR is 90% (range: 79%–97%, median: 92%); the individual nucleotide conservation is shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0057840#pone.0057840.s002" target="_blank">Figure S2</a>. Uppercase letters in the consensus sequence indicate 100% conservation, while lowercase letters indicate the majority nucleotide; “n” indicates no consensus nucleotide could be determined. B: Regulation of Thymidine Kinase minimal promoter (TK minP) by fragments from the region upstream of <i>M. musculus Bmp3</i> in HEK293T cells. Data are mean±SEM normalized to promoter-less control. <i>p</i><0.05 as determined by One-way ANOVA with <i>post hoc</i> Newman-Keuls correction is indicated by “<i>a</i>” versus promoter-less control and by “b” versus CoreECR fragment.</p>