Figure_7.tif (210.39 kB)
Ex vivo response to RMD in multiple longitudinal samples from the same donors.
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posted on 2014-04-10, 02:46 authored by Datsen George Wei, Vicki Chiang, Elizabeth Fyne, Mini Balakrishnan, Tiffany Barnes, Michael Graupe, Joseph Hesselgesser, Alivelu Irrinki, Jeffrey P. Murry, George Stepan, Kirsten M. Stray, Angela Tsai, Helen Yu, Jonathan Spindler, Mary Kearney, Celsa A. Spina, Deborah McMahon, Jacob Lalezari, Derek Sloan, John Mellors, Romas Geleziunas, Tomas CihlarResting CD4 T cells isolated from 2 cART-suppressed HIV-infected patients were treated continuously with RMD or with anti-CD3/CD28 antibodies (AC, activation control) for 7 days. HIV RNA in cell culture supernatants was quantified by COBAS on day 7. Data for a high-responding (A) and a low-responding donor (B) to RMD is shown; each donor was tested at 3 different time points separated by at least 2 weeks (Exp 1–3). VC/NDC, vehicle control/no-drug control. Asterisk (*) indicates no value due to COBAS analysis failure. Dashed lines indicate the limit of HIV quantification by COBAS (20 copies/ml). Data for each individual donor and condition including results of the statistical analysis are summarized in Supplementary Table S4.
