Evaluation of renal collagen deposition using Masson's trichrome stain.

(Blue: collagen [arrow], Pink: cytoplasm; Brown-dark blue: nuclei) and Sirius red stain counter-colored with hematoxylin, (Pink: collagen [arrow], Blue: nuclei). (A) The extent of the renal lesions is represented by the RIF (renal interstitial fibrosis) index. §: P<0.01 compared with SHO group, ???: P<0.01 compared with UUO group, ☆: P>0.05 compared with UUO group. (B) Representative samples of Masson and Sirius red staining for SHO (sham operation), UUO (model group subjected to unilateral ureteral obstruction), PHB⁻ (UUO rats treated with lentivirus carrying siRNA PHB), and PHB⁺ (UUO rats treated with lentivirus carrying PHB) groups; Negative control: UUO rats treated with control viruses.