Fig_2.tif (139.55 kB)
Evaluation of NTR-specific uncaging of NCL probe.
figure
posted on 2015-06-25, 03:40 authored by Anzhelika G. Vorobyeva, Michael Stanton, Aurélien Godinat, Kjetil B. Lund, Grigory G. Karateev, Kevin P. Francis, Elizabeth Allen, Juri G. Gelovani, Emmet McCormack, Mark Tangney, Elena A. Dubikovskaya(A) NCL (20 μM) uncaging by NTR (0.5 μg mL-1) in the presence of NADH (100 μM) was inhibited by dicoumarol (0 to 200 μM). (B) Total luminescence over 30 min from luciferin or NCL (0.25–5 μM) with NADH, luciferase, and NTR, compared with the control (no NTR), *P < 0.001.
