Estimation of the size of the protein aggregates formed in the course of GDH aggregation registered in the regime of heating at the rate of 1°C/min (0.1 M Na-phosphate buffer, pH 7.6).

(A) The initial parts of the dependences of the hydrodynamic radius (Rh) of the protein aggregates on temperature obtained at the following GDH concentrations: (1) 0.1, (2) 0.2, (3) 0.3 and (4) 0.4 mg/ml. (B and C) The dependences of parameter R_h,0 and the reciprocal value of parameter ΔT_2R calculated from Eq. (9) on the GDH concentration, respectively.