Figure_4.tif (5.35 MB)

Enhanced Sensitivity of Csb^m/m/Xpa^−/− Retinal Photoreceptor Cells to Genotoxic Insults

figure

posted on 2013-02-21, 13:04 authored by Ingrid van der Pluijm, George A Garinis, Renata M. C Brandt, Theo G. M. F Gorgels, Susan W Wijnhoven, Karin E. M Diderich, Jan de Wit, James R Mitchell, Conny van Oostrom, Rudolf Beems, Laura J Niedernhofer, Susana Velasco, Errol C Friedberg, Kiyoji Tanaka, Harry van Steeg, Jan H. J Hoeijmakers, Gijsbertus T. J van der Horst

Representative pictures of TUNEL stained retinas of 19-d-old wt, Csb^m/^m, Xpa^−/−, and Csb^m/m/Xpa^−/− mice (right panel), 20 h after exposure of animals to 10 Gy of ionizing radiation, and quantification of the number of TUNEL positive cells in the ONL (left panel). Note the significantly higher number of TUNEL-positive cells in the retina of Csb^m/m/Xpa^−/− mice, as compared to wt and single-mutant littermate controls (p < 0.05). Single asterisks indicate statistically significant differences between unirradiated Csb^m/m/Xpa^−/− and littermate control mice (p < 0.05), double asterisks indicate statistically significant differences between unirradiated and irradiated Csb^m/m/Xpa^−/− mice (p < 0.05).