Endogenous EB1 and EB3 co-localize with the ciliary marker centrin-3 in mouse retinal cryosections.

A: Immunohistochemical localization of EB1 (green) and centrin-3 (red) in mouse retinal cryosections. Nuclei are labelled with DAPI (blue). The DIC (differential interference contrast) image on the left illustrates the laminated retinal cytoarchitecture, labelled as follows: ganglion cell layer (GCL), inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform layer (OPL), outer nuclear layer (ONL), photoreceptor inner segments (IS) and photoreceptor outer segments (OS). B: High magnification of the immunohistochemical localization of EB1 (green) and centrin-3 (red) in photoreceptors. The photoreceptor inner segments (IS) and connecting cilia (cc) are labelled. C: High resolution image of EB1 (green) and centrin-3 (red) in the photoreceptor connecting cilium. The connecting cilium (cc) and basal body (bb) are labelled and an asterisk demarcates the cilia-associated centriole. D: High resolution image of EB3 (red) and centrin-3 (green) in the photoreceptor connecting cilium. The connecting cilium (cc) and basal body (bb) are labelled, and the cilia-associated centriole is demarcated by an asterisk. Scale bars: A, B: 10 μm; C, D: 1 μm.