Figure_12.tif (434.07 kB)
Effects of the supernatants collected after incubation of PBMCs with adefovir, tenofovir, and PMEO-DAPym on the expression of CCR5.
figure
posted on 2013-07-25, 02:07 authored by Jan Balzarini, Graciela Andrei, Emanuela Balestra, Dana Huskens, Christophe Vanpouille, Andrea Introini, Sonia Zicari, Sandra Liekens, Robert Snoeck, Antonín Holý, Carlo-Federico Perno, Leonid Margolis, Dominique ScholsPBMCs were treated with medium alone or with various concentrations of adefovir, tenofovir, or PMEO-DAPym, and the supernatants were collected after 24 h. Then, freshly isolated PBMCs were incubated at 37°C for 1 h with these various supernatants, with medium alone, or with LD78β (control) at 100 ng/ml, 10 ng/ml, and 1 ng/ml. We measured the expression of the CCR5 receptor using flow cytometry with the phycoerythrin-labeled CCR5 (clone 2D7) mAb: shown are the percentages (means ± SEM for two independent experiments) of CCR5+ cells.
