Effects of the K-domain of Dt2 on Dt1 promoter activity.

(A) Diagrammatic Illustration of the conserved motifs in the Dt2 CDS that encode MADS-domain, I-domain, K-domain and C-terminal domain, respectively. (B) GUS staining of the Arabidopsis seedlings with different combination of the transgenes. Proportions of the Dt2 CDS integrated into plasmids for transformation are shown in the (A). (C) Quantitative measurement of GUS activities in the intact seedlings of individual Arabidopsis lines. Doubled-asterisks and asterisks indicate significant differences between compared samples at the levels of p<0.01 and p<0.05 (t-test). (D) Expression levels of the Dt2, Dt2ΔC, Dt2ΔK of under the control of the 35S promoter in the Arabidopsis seedlings and the presence of ProDt1:GUS. The “expression level” of Dt2 in the ProDt1-GUS transgenic line (as a control) was set as 1, and those of Dt2, Dt2ΔC, Dt2ΔK in three corresponding transgenic lines were adjusted accordingly. Values in (D) are shown as mean δ standard errors of the means from three technical replicates.