Figure_3.tif (602.18 kB)
Effects of stable SIRT1 knockdown on mRNA expression of SASP components.
figure
posted on 2015-01-30, 02:58 authored by Tomohisa Hayakawa, Mika Iwai, Satoshi Aoki, Koichi Takimoto, Mitsuo Maruyama, Wakako Maruyama, Noboru MotoyamaA, B, and C, The indicated cells were exposed (or not) to 10Gy of X-irradiation. Total RNA was extracted on the indicated days after irradiation. I IL-8, IL-6, IL-1β, and GRO-α mRNA levels were then determined by quantitative RT-PCR analysis. The levels of the indicated mRNA were normalized to that of GAPDH mRNA and shown as fold induction by 0h. Data are means ± s.d. of triplicates from experiments that were repeated at least twice with similar results.
