Effects of roflumilast on intracellular cAMP levels.

<p>(A) Lung parenchymal explants were incubated for either 20 min or 2h with media and vehicle or with LPS (1 µg/mL) and vehicle or roflumilast at 1 nM or 100 nM. (B) Lung parenchymal explants were exposed to vehicle or roflumilast at 1 nM or 100 nM in the presence or absence of formoterol (10 nM) and with LPS (1 µg/ml) for 20 min. Tissue homogenates were then analyzed for cAMP by EIA. The data shown are the means ± S.E.M. for samples from four different donors. <sup>*</sup><i>p</i>< 0.05 vs. vehicle control (A); <sup>*</sup><i>p</i>< 0.05; <sup>**</sup><i>p</i>< 0.01 vs. LPS, <b><sup>#</sup></b><i>p</i><0.05; <b><sup># #</sup></b><i>p</i><0.01 vs. formoterol and LPS (B).</p>