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Effects of homocysteine on intracellular ROS and mitochondrial membrane potential of BMSCs.

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posted on 2013-05-07, 02:27 authored by Benzhi Cai, Xingda Li, Yang Wang, Yanju Liu, Fan Yang, Hongyang Chen, Kun Yin, Xueying Tan, Jiuxin Zhu, Zhenwei Pan, Baoqiu Wang, Yanjie Lu

(a) Intracellular ROS level was measured in BMSCs treated with homocysteine 30, 100 and 300 µM for 24 h by DCFH-DA staining. The ROS level was gradually increased with the increase of homocysteine concentration. (b) Homocysteine induced an obvious depolarization of mitochondrial membrane potential in BMSCs apoptosis by JC-1 staining.

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