Fig_5.tif (1.43 MB)

Effects of astaxanthin on the regulation of the TNF-α/JNK/p-JNK pathway in mice with ConA-induced acute hepatitis.

figure

posted on 2015-03-11, 03:10 authored by Jingjing Li, Yujing Xia, Tong Liu, Junshan Wang, Weiqi Dai, Fan Wang, Yuanyuan Zheng, Kan Chen, Sainan Li, Huerxidan Abudumijiti, Zheng Zhou, Jianrong Wang, Wenxia Lu, Rong Zhu, Jing Yang, Huawei Zhang, Qin Yin, Chengfen Wang, Yuqing Zhou, Jie Lu, Yingqun Zhou, Chuanyong Guo

(A) The expression of TNF-α and TRAF2 was determined with real-time PCR (n = 8, *P < 0.05 for Oil versus ConA, ^#P < 0.05 for ConA+Astaxanthin (20) versus ConA, ⁺P < 0.05 for ConA+Astaxanthin (40) versus ConA). (B) The levels of proteins TNF-α, TRAF2, JNK, and p-JNK in liver tissue are shown as western blot bands. (C) The expression of TNF-α and p-JNK in hepatic tissues was determined with immunohistochemistry at 8 h (original magnification, 200) and their IODs changed significantly with astaxanthin treatment (n = 8, *P < 0.05 for ConA+Astaxanthin (20) versus ConA, ^#P < 0.05 for ConA+Astaxanthin (40) versus ConA). (D) The levels of proteins ERK, p-ERK, P38 MAPK, and p-P38 MAPK in liver tissue are shown as western blot bands. The relative band densities were calculated (n = 3, *P < 0.05 for Oil versus ConA, ^#P < 0.05 for ConA+Astaxanthin (20) versus ConA, ⁺P < 0.05 for ConA+Astaxanthin (40) versus ConA).