Fig_4.tif (2.17 MB)

Effects of astaxanthin on apoptosis and autophagy in mice with ConA-induced acute hepatitis.

figure

posted on 2015-03-11, 03:10 authored by Jingjing Li, Yujing Xia, Tong Liu, Junshan Wang, Weiqi Dai, Fan Wang, Yuanyuan Zheng, Kan Chen, Sainan Li, Huerxidan Abudumijiti, Zheng Zhou, Jianrong Wang, Wenxia Lu, Rong Zhu, Jing Yang, Huawei Zhang, Qin Yin, Chengfen Wang, Yuqing Zhou, Jie Lu, Yingqun Zhou, Chuanyong Guo

(A) cDNA levels of LC3, Beclin-1, Bcl-2, and Bax were measured with real-time PCR (n = 8, *P < 0.05 for Oil versus ConA, ^#P < 0.05 for ConA+Astaxanthin (20) versus ConA, ⁺P < 0.05 for ConA+Astaxanthin (40) versus ConA). (B) Protein expression of LC3, Beclin-1, Bcl-2, Bax, and caspase 9 was detected with western blotting. (C) Immunohistochemistry was used to detect LC3, Beclin-1, Bcl-2, and caspase 9 (original magnification, 200). The integrated optical densities (IODs) of different the indices are expressed as means ± SD (n = 8, ⁺P < 0.05 for Oil versus ConA, *P < 0.05 for ConA+Astaxanthin (20) versus ConA, ^#P < 0.05 for ConA+Astaxanthin (40) versus ConA). (D) Autophagosome formation was detected in liver tissues with transmission electron microscopy at 8 h (magnification, 10,000). Arrows indicate autophagosomes.