Effects of asm-3 on dauer formation regulation in various mutants defective in thedaf-2 signaling.

(A) Loss of asm-3 enhanced dauer formation of daf-2(e1370) mutants at the semi-permissive temperature 22.5°C. (B) asm-3 mutation greatly enhanced dauer arrest phenotype of age-1(mg305) mutants at 22.5°C. (C) asm-3 mutation did not affect dauer arrest induced by the pdk-1(sa709) mutation at 27°C. The mutant animals carrying sa709 allele formed dauer at 27°C but not at 25°C. (D) asm-3 mutation partially suppressed the dauer arrest phenotype of akt-1(mg306) mutants at 27°C. No dauers at 25°C were observed for the akt-1(mg306) mutant animals with or without the presence of the asm-3(ok1744) allele. (E) asm-3 mutation had no effect on dauer arrest phenotype of daf-7(e1372) mutants at either 22.5°C or 25°C. The asm-3(ok1744) allele by itself did not induce dauer formation at either 22.5°C or 25°C. Error bars indicate standard deviation from triplicates. Details including total worm numbers used in the assay are listed in Table S1.