Effects of IL-9, IL-4 and IFN-γ on wound healing of in vitro injury model of pleural mesothelial cells.

(A) Pleural mesothelial cells was identified by expression of calretinin and sideward scatter (SSC) using flow cytometry, the representative flow cytometric dot-plots are showing expression of IL-9R, IL-4R, and IFN-γR1 on PMCs from tuberculous pleural effusion. (B) Summary dada of percentages of IL-9R⁺, IL-4R⁺, and IFN-γR1⁺ PMCs (n = 5). (C) Microscopic photography after wound induction on a confluent monolayer of PMCs in a time course from 16 to 48 h revealed that wound healing was enhanced by IL-9 or IL-4 and retarded by IFN-γ (Original magnification: ×200). (D) Graphs show relative wound closure over time, based on the wound gap compared with initial wound size. Mean ± SEM of 5 independent experiments. The comparisons were determined by Kruskal-Wallis one-way analysis of variance on ranks, *p<0.05 compared with medium control at the same time points.