Effect of titrated doses of IL-12 or IL-27 as single agent on the repertoire of cytokine secreted by CEA177–189/355–367 specific CD4+ T cells.
(A). CD4+ T cells were cultured with the relevant peptide and irradiated LCL as APC in the presence of increasing concentrations of IL-27 (0-0,1-1-10-100 ng/ml); after 2 days the cytokine release was evaluated by CBA (IL-5 IL-4, and IFN-γ) or ELISA (IL-13). The data for IL-13 are means of duplicate determination±SD. (B). CD4+ T cells were cultured and tested, as described above, in the presence of increasing concentrations of IL-12 (0-5-20 ng/ml). The basal level of cytokines secretion of CD4+ T cells in the presence of LCL only was subtracted from the sample values and was comprised between 0 and 0,018 ng/ml. The data are representative of at least three experiments.