Effect of spacer length in the DNA bridge on the ligation efficiency.^a

Reactions using dCHr8 were performed at 20°C using 3.3 µM [³²P]-labeled dCHr8, 4.1 µM of capture oligo and 4.1 µM of the indicated bridging template. All bridging oligos contained dG in the pairing position across from the tethered dC.