Effect of spacer length in the DNA bridge on the ligation efficiency.a

a

Reactions using dCHr8 were performed at 20°C using 3.3 µM [32P]-labeled dCHr8, 4.1 µM of capture oligo and 4.1 µM of the indicated bridging template. All bridging oligos contained dG in the pairing position across from the tethered dC.

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