Figure_4.tif (310.1 kB)
Effect of siRNA-mediated SPATS2L knockdown on β2-adrenergic receptor levels in HASM cells.
figure
posted on 2012-07-05, 01:07 authored by Blanca E. Himes, Xiaofeng Jiang, Ruoxi Hu, Ann C. Wu, Jessica A. Lasky-Su, Barbara J. Klanderman, John Ziniti, Jody Senter-Sylvia, John J. Lima, Charles G. Irvin, Stephen P. Peters, Deborah A. Meyers, Eugene R. Bleecker, Michiaki Kubo, Mayumi Tamari, Yusuke Nakamura, Stanley J. Szefler, Robert F. Lemanske Jr., Robert S. Zeiger, Robert C. Strunk, Fernando D. Martinez, John P. Hanrahan, Gerard H. Koppelman, Dirkje S. Postma, Maartje A. E. Nieuwenhuis, Judith M. Vonk, Reynold A. Panettieri Jr., Amy Markezich, Elliot Israel, Vincent J. Carey, Kelan G. Tantisira, Augusto A. Litonjua, Quan Lu, Scott T. Weiss(A) Knockdown efficiency of two SPATS2L siRNAs. Quantitative real-time PCR was done on RNAs extracted from HASM cells transfected with control non-targeting (NT) or SPATS2L-specific siRNAs. (B) Western blot analysis of β2AR protein in SPATS2L knockdown HASM cells. Three independent experiments (siRNA transfection and Western blot analysis) were done in HASM cells. The upper panel is a representative of the three Western blots. Quantification of the β2AR protein amount (normalized against the control β-actin protein) from three Western blots is shown in the lower panel.
