Effect of rC-DSP on protein expression levels in PDL and PDLSC cells.

The cells were treated with or without rC-DSP at 7 days. The cells were lysed and protein expressional levels were detected by Western blot assay using the above protein antibodies, respectively. Fifty µg of total cellular lysates were run on 7% SDS-PAGE gels and subsequently electro-blotted. Membranes were blocked with 5% non-fat milk in TBST buffer for 60 min at room temperature and then probed with primary antibodies of a dilution (1:500-1,000) with 1% bovine serum albumin in TBST buffer at 4°C for overnight. After washing, the membranes were incubated with secondary antibodies of a dilution (1:5,000-10,000). Immunoreactivity was determined using ECL chemiluminescence reagent. β-actin was used as an internal control.