Figure_11.tif (337.46 kB)
Effect of inhibitors on SYTOX Green uptake in E. coli MG1655 and its pump deletion mutants.
figure
posted on 2013-02-20, 19:21 authored by Yoshimi Matsumoto, Kohei Hayama, Shouichi Sakakihara, Kunihiko Nishino, Hiroyuki Noji, Ryota Iino, Akihito YamaguchiExponentially growing cells harvested in PBS containing 0.4% glucose were used. Membrane integrity was determined by measuring SYTOX Green uptake after exposure to D13-9001, PAβN, and PMB for 20 min. Fluorescence was determined (Ex/Em: 504/523 nm) after an incubation of 10 min with SYTOX Green in a black 384-well plate with a plate reader.
