Figure_3.tif (547.55 kB)
Effect of anti-miR-148a on cell phenotype.
figure
posted on 2012-04-09, 01:59 authored by Ke Yuan, Zhaorui Lian, Bill Sun, Marcia M. Clayton, Irene O. L. Ng, Mark A. FeitelsonHepG2CAT, HepG2X and HepG2URG11 cells were (A) transiently transfected with anti-miR-148a or (B) stably transduced with recombinant lentivirus encoding anti-miR-148a. In both cases, cell growth was evaluated by MTT assay on days 1, 2 and 3 of culture. All values were normalized to cells treated only with transfection reagent and are presented as a ratio of test:control O.D. values. (C) Serum starved cells were analyzed by flow cytometry on day 3 after the addition of serum. The percentage of cells in G1,S,G2/M phases are indicated in each panel.
