Effect of aeration on the extreme acid survival of W3110, Δ<i>hybC</i>, Δ<i>hycE</i>, and Δ<i>hypF</i>.

<p>The white bars represent cultures grown with aeration to stationary phase in LBK buffered at pH 5 that were diluted 200-fold into LBK pH 2 and exposed for 2 h with aeration at 37°C. The hatched and black bars represent anaerobic cultures grown to stationary phase in LBK buffered at pH 5.5 that were diluted 200-fold into LBK pH 2.5 (hatched) or pH 2 (black) and exposed for 2 h without aeration at 37°C. Aerobic and anaerobic cultures were maintained as stated in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010132#s4" target="_blank">Materials and Methods</a>. Error bars represent SEM, n = 5 or 6.</p>

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