Fig_4.tif (351.89 kB)

Effect of Tudor-SN knockdown on A. phagocytophilum infection.

figure

posted on 2015-07-17, 04:33 authored by Nieves Ayllón, Victoria Naranjo, Ondrej Hajdušek, Margarita Villar, Ruth C. Galindo, Katherine M. Kocan, Pilar Alberdi, Radek Šíma, Alejandro Cabezas-Cruz, Claudia Rückert, Lesley Bell-Sakyi, Mária Kazimírová, Sabína Havlíková, Boris Klempa, Petr Kopáček, José de la Fuente

(A) I. scapularis ISE6 cells were treated with Tudor-SN or Rs86 control dsRNAs and infected with A. phagocytophilum NY18. A. phagocytophilum DNA levels were characterized by msp4 real-time PCR normalizing against tick 16S rDNA. Normalized Ct values were compared between groups by Student's t-test with unequal variance and were not statistically different between Tudor-SN and Rs86 dsRNA-treated cells (P = 0.05; N = 4 wells per treatment). (B) I. scapularis female ticks were injected with Tudor-SN or Rs86 control dsRNAs and infected with A. phagocytophilum NY18 by feeding on an infected sheep. A. phagocytophilum DNA levels were characterized in tick guts and salivary glands by msp4 real-time PCR normalizing against tick 16S rDNA. Normalized Ct values were compared between groups by Student's t-test with unequal variance and were not statistically different between Tudor-SN and Rs86 dsRNA-injected ticks (P = 0.05; N = 10 ticks per group).