Effect of MS-275 on TGF-beta1-induced expression of collagen 1, alpha-SMA, fibronectin and acetyl-histone H3 in cultured renal interstitial fibroblasts.

Serum-starved NRK-49F cells were incubated with 2 ng/ml TGF-beta1 for 24 h in the absence or presence of MS-275 (0–2 µM). Cell lysates were subject to immunoblot analysis with antibodies to the indicated proteins (A). Representative immunoblots from three or more experiments are shown. Expression levels of the indicated proteins were quantified by densitometry and normalized with GAPDH (B, C, D). Data are represented as the mean ± SEM. Means with different superscript letters are significantly different from one another (P<0.05).