Effect of LTB<sub>4</sub>- or cysLT-receptor antagonists on macrophage phagocytosis and fungicidal activity against <i>P. brasiliensis</i>.

<p>Peritoneal WT macrophages were treated with vehicle, LTB<sub>4</sub> receptor antagonist CP-105,696 (CP) (1 mM) or cysteinyl-LT receptor antagonist montelukast (MK) (10 mM) for 30 min before the addition of <i>P. brasiliensis</i> yeasts. Phagocytic index (A) and intracellular killing (B) were calculated 24 h after incubation. Killing assay was normalized according initial ingestion (PI) and presented as <i>P. brasiliensis</i> survival. The supernatants were harvested and nitrite concentrations (C) were measured. (D) ROS concentrations were determined by mean fluorescence intensity (DHR-123) in macrophage cultures after infection. Each value represents the mean ± SEM of triplicate cultures from two independent experiments. Symbol * represents significant difference (*p<0.05, **p<0.01, ***p<0.001) compared to control group (macrophages that were treated with vehicle and infected with <i>P. brasiliens</i> yeasts). Symbol <sup>†</sup> represents significant difference (<sup>†</sup>p<0.05, <sup>†††</sup>p<0.001) compared to IFN-γ control group (macrophages that were activated with IFN-γ, treated with vehicle and infected with <i>P. brasiliens</i> yeasts). Symbol <sup>‡</sup> represents significant difference (<sup>‡‡</sup>p<0.01) between the same experimental group compared to different culture conditions.</p>