Effect of LTB₄- or cysLT-receptor antagonists on macrophage phagocytosis and fungicidal activity against P. brasiliensis.

Peritoneal WT macrophages were treated with vehicle, LTB₄ receptor antagonist CP-105,696 (CP) (1 mM) or cysteinyl-LT receptor antagonist montelukast (MK) (10 mM) for 30 min before the addition of P. brasiliensis yeasts. Phagocytic index (A) and intracellular killing (B) were calculated 24 h after incubation. Killing assay was normalized according initial ingestion (PI) and presented as P. brasiliensis survival. The supernatants were harvested and nitrite concentrations (C) were measured. (D) ROS concentrations were determined by mean fluorescence intensity (DHR-123) in macrophage cultures after infection. Each value represents the mean ± SEM of triplicate cultures from two independent experiments. Symbol * represents significant difference (*p<0.05, **p<0.01, ***p<0.001) compared to control group (macrophages that were treated with vehicle and infected with P. brasiliens yeasts). Symbol ^† represents significant difference (^†p<0.05, ^†††p<0.001) compared to IFN-γ control group (macrophages that were activated with IFN-γ, treated with vehicle and infected with P. brasiliens yeasts). Symbol ^‡ represents significant difference (^‡‡p<0.01) between the same experimental group compared to different culture conditions.