Effect of LPAATβ on tumor growth in the orthotopic model of osteosarcoma.

<p><b>A</b>. Xenogen IVIS 200 bioluminescence imaging of orthotopic tumor growth. Athymic nude mice were periosteally injected at proximal tibia with 143B cells infected with AdR-LPAATβ, AdRFP, or AdR-siLPAATβ for 15 h (10<sup>6</sup> cells/injection). Animals were subjected to Xenogen imaging at the indicated time points. Representative imaging results at 37 days post injection are shown. <b>B</b>. Quantitative analysis of Xenogen imaging signal intensity (photons/sec/cm<sup>2</sup>/steradian) over the time after injection. <b>C</b>. Tumor growth as determined by tumor volume. The dimensions of injection sites were measured roughly every 3 days during the course of study. Tumor volumes (mm<sup>3</sup>) were calculated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0014182#s2" target="_blank">Methods</a>. “*” <i>p-value <0.05</i>, “**” <i>p</i>-<i>value <0.001</i>.</p>