Effect of IFNAR1 deficiency on the Th2-related response.

WT and IFNAR1KO mice were infected intratracheally with C. neoformans. (A) IL-4, IL-5, and IL-13 production in the lung homogenates was measured on day 3, 7, 14, and 28 after infection. Each column represents the mean ± SD of five to six mice. (B) LN cells obtained on day 7 post-infection were stimulated with indicated doses of C. neoformans or ConA for 48 h, and production of IL-4 was measured. Each column represents the mean ± SD of triplicate cultures. The lung leukocytes (C) and LN cells (D) were prepared on day 7 post-infection. Expression of IL-4 in CD3⁺ CD4⁺ T cells was analyzed using flow cytometry and the number of IL-4⁺ CD3⁺ CD4⁺ T cells was calculated. Each column represents the mean ± SD of five mice. Experiments were repeated twice with similar results and the representative data are shown. NS, not significant; *, p < 0.05.