Effect of 2-DG on T-cell populations.

(A) 2-DG had a differential and dose dependent effect on CD4⁺ (decrease) and CD8⁺ (increase) T-cell subpopulations reflected in reduced (B) CD4⁺/CD8⁺ ratios. Lymphocytes from the spleen of control and 2-DG fed EAC tumor bearing mice were analyzed by flowcytometry to quantitate percentages of CD4⁺ and CD8⁺ populations. Also the effect of 2-DG administration on the (C) CD4⁺CD25⁺ Treg cell population was determined in the peripheral circulation of non tumor bearing and EAC tumor bearing host by flowcytometry. 2-DG prevents tumor-induced augmentation of CD4⁺CD25⁺ Treg cells in tumor-bearing mice compared to no alteration in the non tumor bearing mice. *, p < 0.05; **, p < 0.01; ***, p < 0.001