Early response to DNA damage simulated using the kinetic parameter sets derived from OSC1P1 and OSC2P1.

Simulations were based on the model in Figure 2-B with kinetic parameter sets derived from (A) OSC1P1 and (B) OSC2P1. Maximum level of mRNA (species 15) was quantified during early DNA damage response (0-3 h) as a function of increasing basal production rate constant of Mdm2 (k6) in the presence of different levels of MdmX. The green line with open circles (also indicated as 1 fold) corresponds to a predicted basal production rate of MdmX from the survey of kinetic parameter space. The basal production rate of MdmX was varied from 0 to 100 fold of its predicted value.