Figure_7.tif (8.43 MB)

E-cadherin modulation by soluble IL-15 controls epithelial-mesenchymal transition on renal epithelial cells.

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posted on 2013-02-20, 08:47 authored by Julien Giron-Michel, Sandy Azzi, Krystel Khawam, Erwan Mortier, Anne Caignard, Aurore Devocelle, Silvano Ferrini, Michela Croce, Hélène François, Lola Lecru, Bernard Charpentier, Salem Chouaib, Bruno Azzarone, Pierre Eid

A) Immunofluorescence of cell–cell adhesion molecules show that IL-15 favors epithelial-mesenchymal transition (EMT) on RCC7, whereas it preserves the EMT commitment of RPTEC. The medium culture of RPTEC was not changed in order to induce the EMT process. Cells stimulated or not with 10 pg/ml of rhIL-15 for 5 days, were fixed and stained using standard immunofluorescence procedures with Abs against epithelial (cytokeratins and ZO-1) and mesenchymal markers (F-actin, ASO2 and vimentin). Similar results were obtained using different RCC (RCC5, RCC8) and RPTEC batches. B) After 48 h, transfected RCC were treated for an additional 48 h with 10 pg/mL of rhIL-15 before evaluating the epithelial (cytokeratins) and mesenchymal (vimentin) markers expression by flow cytometry. RhIL-15 induced EMT was counterbalanced only in IL-2Rγ/JAK3 co-transfected RCC. Mean fluorescence intensity values for each marker are shown in each histogram. Results are representative of three experiments.