ELISpot assay for IL-4 secretion induced by GPR120 agonist.

(A) IL-4 ELISpot assay was performed on eosinophils following stimulation with GW9508 (100 μM) or vehicle for 18 h. A23187 (0.5 μM) was used for a positive control. Developed spots represent the number of IL-4 secreted from human eosinophils. Image was taken by stereoscopic microscope. (B) Developed spots were counted by a single investigator in a coded manner. Data are expressed as the mean of four experiments ± SEM from different donors. *p<0.05 vs control.