Figure_5.tif (3.19 MB)
EGF induces EMT only in cells with EGFR mutation and TWIST1 expression (protein level).
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posted on 2012-01-17, 00:46 authored by Karine Pallier, Anatole Cessot, Jean-Francois Côté, Pierre-Alexandre Just, Aurélie Cazes, Elizabeth Fabre, Claire Danel, Marc Riquet, Mojgan Devouassoux-Shisheboran, Stéphane Ansieau, Alain Puisieux, Pierre Laurent-Puig, Hélène BlonsA. Assessment of TWIST1, CDH2, VIM, CDH1 and JUP expression by western blotting after addition of EGF for a 24 h or 48 h period of time on HCC827 (EGFR mutated, TWIST1 not expressed) and H1650 (EGFR mutated, TWIST1 expressed). B. Representative photomicrographs of TWIST1, CDH2, VIM, CDH1 and JUP expression analyzed by immunofluorescence after EGF treatment (25 ng/mL).
