E2F6-interacting proteins identified from a yeast two-hybrid screen.

2012-10-17T02:17:51Z (GMT) by Janet Y. Leung Joseph R. Nevins

Clones identified from the yeast two-hybrid screen were tested for an interaction with E2Fs1–4 and E2F6 in yeast. Full length human E2Fs 1–4 and E2F6 were cloned into pGBT9B as described in Materials and Methods and the resulting plasmid, along with DP1, was transformed into yeast to test for an interaction with the indicated proteins.

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Ectopic expression of E2F4 was able to immunoprecipitate ectopically expressed BRG1 in T98G cells even though an interaction was not observed in yeast.