E1 homozygous mutant flies exhibit dramatic motor impairment and reduced lifespan.

(A) Uba1^B1/Uba1^B1 homozygous flies and Uba1^B1/Uba1^B2 flies demonstrate a dramatic reduction in the ability to climb compared to wild-type +/+ flies. Flies were tested for the ability to climb 4 cm in 5 seconds. Trials of age-matched flies were tested 5 times in small groups of typically 10–15 flies. The age, gender, and total number of flies tested for each genotype is indicated. (B) Climbing assays for wild-type +/+ flies for flies aged 5 days to 55 days. Both males (solid line) and females (dashed line) were tested. The total number of flies tested for each gender and time point is indicated above each data point. Standard Deviation for each data point based on five replicate trials for each genotype is indicated by error bars for A–B. (C, E) Graph showing the percentage of (C) male or (E) female flies surviving versus time in days for wild-type (+/+, open circles) control flies or Uba1^B1/Uba1^B1 homozygous flies (open squares) over time in days. Uba1^B1/Uba1^B1 homozygous flies show a dramatic decrease in lifespan compared to wild-type controls. (D, F) Table summarizing the average ± s.e.m. and median lifespans and the number of flies followed (N) for each of the genotypes graphed in (C, F) respectively.