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Video S1 - Dynamic, Nondestructive Imaging of a Bioengineered Vascular Graft Endothelium

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posted on 2013-04-09, 02:20 authored by Bryce M. Whited, Matthias C. Hofmann, Peng Lu, Yong Xu, Christopher G. Rylander, Ge Wang, Etai Sapoznik, Tracy Criswell, Sang Jin Lee, Shay Soker, Marissa Nichole Rylander

Dynamic imaging of graft endothelialization. A full-vessel scaffold was seeded with GFP-ECs and a 500 µm×250 µm section of the lumen was sequentially imaged at 2 hr intervals for a 3-day period. The video shows the scaffold lumen and corresponding plot of GFP-EC coverage over time. In this video, GFP-ECs are actively migrating along the surface of the lumen and each image can be analyzed to provide a real-time assessment of EC coverage.

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