Drug-induced demethylation patterns in HL60 myeloid leukemia cells.

Methylation changes in HL60 cells treated for 24 h with AZA (A) or DAC (B); blue dots and numbers represent demethylated CGs (DB≤−0.2). C, D, Boxplots show demethylation efficiency indicated by DB values in AZA- and DAC-treated HCT116 cells dependent on CG association with CGIs; black lines denote medians, notches the standard errors, boxes the interquartile range, and whiskers the 2.5th and 97.5th percentiles. Boxplots show demethylation efficiencies as a function of degree of CG methylation in E, AZA- and F, DAC-treated HL60 cells. Methylation levels were grouped in 10% intervals from 0 to 100% methylation; black marks denote medians, boxes the interquartile range, and whiskers the 2.5th and 97.5th percentiles.