Dorsomorphin and BMP2 RNAi inhibit osteogenic differentiation of MC3T3-E1 cells induced by FSS.

(A) DM and (B) BMP2 RNAi (si-BMP2) attenuated transcription of BMP2 gene induced by FSS. (C) DM and (D) si-BMP2 suppressed BMP2 protein secretion induced by FSS. DM and si-BMP2 repressed transcription of osteogenic differentiation markers including (E & F) ALP, (G & H) RUNX2 and (I & J) SP7. (K) DM and (L) si-BMP2 inhibited FSS-induced ALP activity. (M) DM and (N) si-BMP2 canceled mineralization of ECM at day 12. (NC: Scramble sequence, negative control in transfection. Scale bar: 50 µm. Data were shown as mean ± SD. n = 3. *, FSS group versus control group, or NC + FSS group versus NC group; #, FSS + DM group versus FSS group, or si-BMP2 + FSS group versus si-BMP2 group. #, P<0.05; ** and ##, P<0.01; *** and ###, P<0.001.)