Dorsomorphin and <i>BMP2</i> RNAi inhibit osteogenic differentiation of MC3T3-E1 cells induced by FSS.

<p>(A) DM and (B) <i>BMP2</i> RNAi (si-<i>BMP2</i>) attenuated transcription of <i>BMP2</i> gene induced by FSS. (C) DM and (D) si-<i>BMP2</i> suppressed BMP2 protein secretion induced by FSS. DM and si-<i>BMP2</i> repressed transcription of osteogenic differentiation markers including (E & F) <i>ALP</i>, (G & H) <i>RUNX2</i> and (I & J) <i>SP7</i>. (K) DM and (L) si-<i>BMP2</i> inhibited FSS-induced ALP activity. (M) DM and (N) si-<i>BMP2</i> canceled mineralization of ECM at day 12. (NC: Scramble sequence, negative control in transfection. Scale bar: 50 µm. Data were shown as mean ± SD. <i>n</i> = 3. *, FSS group versus control group, or NC + FSS group versus NC group; #, FSS + DM group versus FSS group, or si-<i>BMP2</i> + FSS group versus si-<i>BMP2</i> group. #, <i>P</i><0.05; ** and ##, <i>P</i><0.01; *** and ###, <i>P</i><0.001.)</p>