Disturbance of the neural progenitor zone altered the pattern of vdINVP.

(A) Schematic illustration of pBI-based expression vectors. pBI-vector and pCAGGS-tTA were used without Dox, the combination that retained expression of electroporated DNAs. (B) Control electroporation with EGFP. (C-E) DN-RhoA was electroporated unilaterally into the neural tube. Thick transverse sections were prepared from highlighter ink-infused embryos and co-stained for markers indicated, followed by confocal microscopy. Top and bottom panels are identical views. (F, G) Similar experiments using CA-Rac1. Arrows: vdINVP misdirected into a lumen-facing region. (B-E) E5.5/HH27. (F, G) E5/HH26 Scale bars: 100 μm.