Differential specificity of Dictyostelium Sfp (DiSfp) towards mycobacterial PKS2 and kinetic analysis.
Gel-binding assays were set up with DiAcpS and DiSfp, taking [1-14C] acetyl-CoA (A-CoA), [1-14C] hexanoyl-CoA (H-CoA) and [1-14C] lauroyl-CoA (L-CoA) as co-substrates. A, Panels show the autoradiogram of SDS-PAGE in which mycobacterial PKS2 was labeled by different acyl CoA substrates and PPTases as indicated. B, Gel-based kinetic analysis of DiSfp with respect to acetyl CoA. Concentration of acetyl CoA was varied from 5 µM to 45 µM.