Figure_2.tif (800.71 kB)
Diagram of the lentivirus vector shuttle plasmids pLV.pA+.GS.DsRed and pLV.pA+.GS.Luc.
figure
posted on 2013-02-21, 01:06 authored by Manuel A. F. V. Gonçalves, Josephine M. Janssen, Maarten Holkers, Antoine A. F. de VriesGrey box with broken arrow, 5′ hybrid long terminal repeat (LTR) containing Rous sarcoma virus and HIV-1 sequences; Grey box without broken arrow, 3′ SIN LTR; ψ, HIV-1 packaging signal; Cyan arrowheads, FLP recognition target (FRT) sites; orange box with broken arrow, human glyceraldehyde-3-phosphate dehydrogenase (hGAPDH) gene promoter; Large and small green boxes, rabbit β-globin and murine metallothionine gene pAs (rBGpA and mMTpA, respectively); red box, DsRed.T4-N1 ORF from Discosoma sp. (DsRed); yellow box, luciferase ORF from Photinus pyralis (PpLuc). All genetic elements are drawn to scale.
