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Determination of the optimal number of reference genes required for effective normalization.

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posted on 2014-08-12, 04:03 authored by Xia Yan, Xicun Dong, Wen Zhang, Hengxia Yin, Honglang Xiao, Peng Chen, Xiao-Fei Ma

Pairwise variation (Vn/Vn+1) analysis between the normalization factors (NFn and NFn+1) was performed by the geNorm program to determine the optimal number of reference genes and was carried out for qPCR data normalization in various sample pools.

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    histone H 2Apcrhgef10 candidate reference genesreference Gene Selectionpolymerase chain reactioneukaryotic initiation factor 4CYCLeifReference genes4aReaumuria soongoricaXGG

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