Figure_10.tif (1.3 MB)
Detection of anti-C. jejuni pentabodies (FlagV1P) in different sections of the chicken intestinal tract.
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posted on 2013-12-31, 05:45 authored by Ali Riazi, Philippa C. R. Strong, Russell Coleman, Wangxue Chen, Tomoko Hirama, Henk van Faassen, Matthew Henry, Susan M. Logan, Christine M. Szymanski, Roger MacKenzie, Mehdi Arbabi GhahroudiSeparate chickens were gavaged with the flagella-specific pentabody according to the schedule that was followed in our chicken colonization studies. Intestinal fluids were collected from the cecum, ileum, jejunum and duodenum and used in 2-fold serial dilutions {1/2 (no. 2)-1/2048 (no. 12)} in ELISA using anti-verotoxin antibody to capture the pentabodies and anti-His-HRP antibody conjugates for their detection. Chickens were gavaged with 1 mg of FlagV1P.
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Agricultural biotechnologyAnimal managementBiochemistrybiotechnologygeneticsimmunologyimmunoglobulinsImmunologic techniquesmicrobiologyimmunityimmunotherapyMolecular cell biologyClinical immunologyInfectious diseasesBacterial diseasescampylobacterVeterinary diseasesZoonotic diseasesFoodborne diseasesVeterinary microbiologyVeterinary pathologypentabodiessectionsintestinal
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